Coding

Part:BBa_K365013:Design

Designed by: RENAULT Renaud   Group: iGEM10_ESBS-Strasbourg   (2010-10-10)


ClpX trimer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 699
    Illegal BglII site found at 1863
    Illegal BglII site found at 3027
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See subparts.


Source

∆N-ClpX was initially extracted from E. coli genome using colony PCR, then we deleted some restriction sites. We then used standard assembly protocols.

References